宮崎大学獣医学部 関口研究室 宮崎大学 農学部 獣医学科
産業動物伝染病防疫学研究室

研究業績

私たちの研究室では、動物の命を守ること、
食の安全・安心・安定供給を守ることを目指して、
家畜防疫に関する研究に取り組んでいます。

2023年

  • Notsu, K., El Daous, H., Mitoma, S., Wu, X., Norimine, J., & Sekiguch, S. (2023). Identifying Pathogen and Allele Type Simultaneously in a Single Well Using Droplet Digital PCR. mSphere, e0049322. Advance online publication. https://doi.org/10.1128/msphere.00493-22

2022年

  • Notsu, K., Inoue, Y., Masuda, T., Inoue, M., Mitoma, S., Wu, X., Norimine, J., & Sekiguch, S. (2022). A survey of bovine leukemia virus resistant bovine leukocyte antigen (BoLA)-DRB3*009:02 allele-carrying Japanese Black cattle in two prefectures in Japan. The Journal of veterinary medical science, 84(11), 1457–1460. https://doi.org/10.1292/jvms.22-0285
  • Ota, R., Iwaki, E., Sakai, K., Haraguchi, T., Kaneko, Y., Sekiguch, S., Yamaguchi, R., & Naganobu, K. (2022). Dural Changes Induced by an Ultrasonic Bone Curette in an Excised Porcine Spinal Cord. Veterinary sciences, 9(11), 601. https://doi.org/10.3390/vetsci9110601
  • Mai, N. T., Tuyen, L. A., Van Truong, L., Huynh, L., Huong, P., Hanh, V. D., Anh, V. V., Hoa, N. X., Vui, T. Q., & Sekiguch S. (2022). Early-phase risk assessments during the first epidemic year of African swine fever outbreaks in Vietnamese pigs. Veterinary medicine and science, 10.1002/vms3.852. Advance online publication. https://doi.org/10.1002/vms3.852
  • Mai, T. N., Sekiguch S., Huynh, T., Cao, T., Le, V. P., Dong, V. H., Vu, V. A., & Wiratsudakul, A. (2022). Dynamic Models of Within-Herd Transmission and Recommendation for Vaccination Coverage Requirement in the Case of African Swine Fever in Vietnam. Veterinary sciences, 9(6), 292. https://doi.org/10.3390/vetsci9060292
  • Duong, H. D., Taniguchi, Y., Takashima, Y., Sekiguch S., Aye, K. M., Ahmadi, P., Bui, L. K., Irie, T., Nagayasu, E., & Yoshida, A. (2022). Diagnostic value of recombinant nanoluciferase fused Toxoplasma gondii antigens in Luciferase-linked Antibody Capture Assay (LACA) for Toxoplasma infection in pigs. The Journal of veterinary medical science, 10.1292/jvms.22-0099. Advance online publication. https://doi.org/10.1292/jvms.22-0099
  • Notsu, K., El Daous, H., Mitoma, S., Norimine, J., & Sekiguch S. (2022). A pooled testing system to rapidly identify cattle carrying the elite controller BoLA-DRB3*009:02 haplotype against bovine leukemia virus infection. HLA, 99(1), 12–24. https://doi.org/10.1111/tan.14502

2021年

  • Daous, H. E., Mitoma, S., Elhanafy, E., Thi Nguyen, H., Thi Mai, N., Notsu, K., Kaneko, C., Norimine, J., & Sekiguch S. (2021). Relationship between Allelic Heterozygosity in BoLA-DRB3 and Proviral Loads in Bovine Leukemia Virus-Infected Cattle. Animals : an open access journal from MDPI, 11(3), 647. https://doi.org/10.3390/ani11030647
  • Mitoma, S., Carr, B. V., Harvey, Y., Moffat, K., Sekiguch S., Charleston, B., Norimine, J., & Seago, J. (2021). The detection of long-lasting memory foot-and-mouth disease (FMD) virus serotype O-specific CD4+ T cells from FMD-vaccinated cattle by bovine major histocompatibility complex class II tetramer. Immunology, 164(2), 266–278. https://doi.org/10.1111/imm.13367
  • Yamagami, T., Miyama, T., Toyomaki, H., Sekiguch S., Sasaki, Y., Sueyoshi, M., & Makita, K. (2021). Analysis of the effect of feedback feeding on the farm-level occurrence of porcine epidemic diarrhea in Kagoshima and Miyazaki Prefectures, Japan. The Journal of veterinary medical science, 83(11), 1772–1781. https://doi.org/10.1292/jvms.21-0343
  • Wu, X., Kitahara, G., Suenaga, T., Naramoto, K., Sekiguch S., Goto, Y., & Osawa, T. (2021). Association of intrauterine presence of Lactobacillus spp. with inflammation and pathogenic bacteria in the uterus in postpartum dairy cows. The Journal of reproduction and development, 67(6), 340–344. https://doi.org/10.1262/jrd.2021-023
  • Tangkonda, E., Kubo, M., Sekiguch S., Shinki, T., Sasaki, S., Yamada, K., Taniguchi, T., Vetchapitak, T., & Misawa, N. (2021). Work-related increases in titer of Campylobacter jejuni antibody among workers at a chicken processing plant in Miyazaki prefecture, Japan, independent of individual ingestion of edible raw chicken meat. The Journal of veterinary medical science, 83(8), 1306–1314. https://doi.org/10.1292/jvms.21-0244
  • Kabali, E., Pandey, G. S., Munyeme, M., Kapila, P., Mukubesa, A. N., Ndebe, J., Muma, J. B., Mubita, C., Muleya, W., Muonga, E. M., Mitoma, S., Hang'ombe, B. M., Wiratsudakul, A., Ngan, M. T., Elhanafy, E., Daous, H. E., Huyen, N. T., Yamazaki, W., Okabayashi, T., Abe, M., … Sekiguch S. (2021). Identification of Escherichia coli and Related Enterobacteriaceae and Examination of Their Phenotypic Antimicrobial Resistance Patterns: A Pilot Study at A Wildlife-Livestock Interface in Lusaka, Zambia. Antibiotics (Basel, Switzerland), 10(3), 238. https://doi.org/10.3390/antibiotics10030238
  • Mitoma S, El-Khaiat HM, Uto T, Sato K, Sekiguch S, Norimine J. Characterization of bovine interleukin-2 stably expressed in HEK-293 cells. J Vet Med Sci. 83(1):134-141. doi:10.1292/jvms.20-0423. 2021

2020年

  • Notsu K, Wiratsudakul A, Mitoma S, Daous H, Kaneko C, El-Khaiat H, Norimine J, Sekiguch S. Quantitative risk assessment for the introduction of bovine leukemia virus-infected cattle using a cattle movement network analysis. pathogens. 9, 903, https://doi.org/10.3390/pathogens9110903. 2020
  • Mai T.N, Yamazaki Y, Bui T.P, Nguyen V.D, Le Huynh T.M. Mitoma S, El Daous H, Kabali E, Norimine J, Sekiguch S. A descriptive survey of porcine epidemic diarrhea in pig populations in northern Vietnam. Tropical Animal Health and Production. DOI: 10.1007/s11250-020-02416-1. 2020
  • Takatsuka K, Sekiguch S, Yamaba H, Aburada K, Mukunoki M, Okazaki N. FMD-VS: A virtual sensor to index FMD virus scattering. PLOS ONE. https://doi.org/10.1371/journal.pone.0237961. 2020
  • Mai T.N., Bui T.P., Le Huynh T.M., Sakai Y., Mitoma S., El Daous H, Fahkrajang W., Norimine J, Sekiguch S. Evaluating the Risk Factors for Porcine Epidemic Diarrhea Virus Infection in an Endemic Area of Vietnam. Front. Vet. Sci. doi: 10.3389/fvets.2020.00433. 2020
  • Furutani A, Sekiguch S, Sueyoshi M, Sasaki Y. Assessment of abortion risk of sows on Japanese commercial farms infected with porcine epidemic diarrhea virus. Animal Science Journal. DOI: 10.1111/asj.13377. 2020
  • Lei Z, Haga T, Obara H, Sekiyama Hi, Sekiguch S, Hombu A, Fujihara M, Lei L, Hsu S, Zhang X, Ishitsuka I, Atagi Y, Sato T, Sugiura K. A questionnaire survey of the illegal importation of pork products by air travelers into Japan from China and exploration of causal factors. Preventive Veterinary Medicine. doi.org/10.1016/j.prevetmed.2020.104947. 2020
  • El Daous H, Mitoma S, Elhanafy E, Thi Nguyen H, Thi Mai N, Hara A, Duangtathip K, Takezaki Y, Kaneko C, Norimine J, Sekiguch S. Establishment of a novel diagnostic test for Bovine leukaemia virus infection using direct filter PCR. Transbound Emerg Dis. 8:10.1111/tbed.13506. doi: 10.1111/tbed.13506. 2020
  • Ong BHE, Hidaka Y, Kaneko Y, Yamamoto S, Mizutani S, Sekiguch S, Torisu S, Naganobu K. Effects of a single-bolus bupivacaine injection into the coccygeal spinal canal of rabbits. J Vet Med Sci. 18; 82: 197-203. doi: 10.1292/jvms.19-0555. 2020

2019年

  • Myint O, Yoshida A, Sekiguch S, Nguyen VD, Fuke N, Uda ZI, Hirai T and Yamaguchi R. Development of indirect enzyme-linked immunosorbent assay for detection of porcine epidemic diarrhea virus specific antibodies (IgG) in serum of naturally infected pigs. BMC Veterinary Research. 15(1), 409. https://doi.org/10.1186/s12917-019-2123-2. 2019
  • Arikawa G, Fujii Y, Abe M, Mai TN, Mitoma S, Notsu K, Nguyen TH, Elhanafy E, Daous H, Kabali E, Norimine J and Sekiguch S. Meteorological factors affecting the risk of transmission of HPAI in Miyazaki, Japan. Vet Record Open. 6:e000341. doi:10.1136/vetreco-2019-000341 2019
  • Ueno Y, Uemura R, Niwa H, Higuchi T, Sekiguch S, Sasaki Y and Sueyoshi M. Total serum protein reference value for clinical diagnostic index of equine proliferative enteropathy. J Equine Sci. 30: 63-67. 2019
  • Agah AM, Notsu K, EL-Khaiat H, Arikawa G, Kubo M, Mitoma S, Okabayashi T, Mekata H, Elhanafy E, Daous EH, Mai TN, Nguyen TH, Isoda N, Sakoda Y, Norimine J and Sekiguch S. Slaughterhouse survey for detection of bovine viral diarrhea infection among beef cattle in Kyushu, Japan. J Vet Med Sci. 81: 1450-1454. 2019
  • Furutani A, Sekiguch S, Sueyoshi M, Sasaki Y. Effect of intervention practices to control the porcine epidemic diarrhea (PED) outbreak during the first epidemic year (2013–2014) on time to absence of clinical signs and the number of dead piglets per sow in Japan. Prev Vet Med. 169: 104710. 2019
  • Sasaki Y, Sekiguch S, Toyomaki H, Makita K and Sueyoshi M. Assessment of the economic impact of porcine epidemic diarrhea (PED) epidemic in the southern Kyushu, Japan. Journal of Veterinary Epidemiology. In press. 2019
  • Naganobu K, Nakajima A, Kaneko Y, Torisu S, Sekiguch S and Chuman H. Hydroxyl radical formation in lactated Ringer’s solution and BSS Plus® intraocular irrigating solution. Japanese Journal of Veterinary Research. 67(2). 2019

2018年

  • Notsu K, Hashida S, Mitoma S, Kubo M, Arikawa G, Agah AM, El-Khaiat H, Mai TN, Nguyen H, Elhanafy E, Daous H, Norimine J, Sekiguch S. Assessment of hematological parameters and carcass weight in bovine leukemia virus infection in slaughtered beef cattle. Journal of Veterinary Epidemiology. 22: 43-48. 2018
  • Wiratsudakul A, Sekiguch S. The implementation of cattle market closure strategies to mitigate the foot-and-mouth disease epidemics: A contact modeling approach. Research in Veterinary Science. 121: 76–84. 2018
  • Koike N, Mai TN, Shirai M, Kubo M, Hata K, Marumoto N, Watanabe S, Sasaki Y, Mitoma S, Notsu K, Okabayashi T, Wiratsudakul A, Kabali E, Norimine J, Sekiguch S. Detection of neutralizing antibody against porcine epidemic diarrhea virus in subclinically infectedfinishing pigs. J Vet Med Sci. doi:10.1292/jvms.18-0132. 2018
  • Probert WJM, Jewell CP, Werkman M, Fonnesbeck CJ, Goto Y, Runge MC, Sekiguch S, Shea K, Keeling MJ, Ferrari MJ, Tildesley MJ. Real-time decision-making during emergency disease outbreaks. PLoS Comput Biol. 14(7):e1006202. doi: 10.1371/journal.pcbi.1006202. eCollection 2018
  • Sudaryatma PE, Nakamura K, Mekata H, Sekiguch S, Kubo M, Kobayashi I, Subangkit M, Goto Y, Okabayashi T. Bovine respiratory syncytial virus infection enhances Pasteurella multocida adherence on respiratory epithelial cells. Vet Microbiol. 220:33-38. doi: 10.1016/j.vetmic.2018.04.031. 2018
  • Mai T.N., Nguyen V.D., Yamazaki W., Okabayashi T., Mitoma S., Notsu K., Sakai Y., Yamaguchi R., Norimine J, Sekiguch S. Development of pooled testing system for porcine epidemic diarrhea using real-time fluorescent reverse-transcrition loop-mediated isothermal amplification assay. BMC Vet. Res. doi: 10.1186/s 12917-018-1498-9. 2018.
  • Mekata H, Yamamoto M, Hayashi T, Kirino Y, Sekiguch S, Konnai S, Horii Y, Norimine J. Cattle with a low bovine leukemia virus proviral load are rarely an infectious source. Japanese Journal of Veterinary Research, 66: 157-163. 2018
  • Mekata H, Yamamoto M, Kirino Y, Sekiguch S, Konnai S, Horii Y, Norimine J. New hematological key for bovine leukemia virus-infected Japanese Black cattle. J. Vet. Med. Sci. doi: 10.1292/jvms.17-0455. 2018
  • Toyomaki H, Sekiguch S, Sasaki Y, Sueyoshi M, Makita K. Factors associated with farm-level infection of porcine epidemic diarrhea during the early phase of the epidemic in Japan in 2013 and 2014. Preventive Veterinary Medicine. 150:77-85. 2018

2017年

  • Sasaki Y, Toyomaki H, Sekiguch S, Sueyoshi M, Makita K, Otake S, Perez A, Alvarez J. Spatial dynamics of porcine epidemic diarrhea (PED) spread in the southern Kyushu, Japan. Prev Vet Med. 144:81-88 2017
  • Sekiguch S, Presi P, Omori R, Staerk K, Schuppers M, Isoda N, Yoshikawa Y, Umemura T, Nakayama H, Fujii Y, Sakoda Y. Evaluation of bovine viral diarrhea virus control strategies in dairy herds in Hokkaido, Japan, using stochastic modelling. Transbound Emerg Dis. doi: 10.1111/tbed.12693. 2017
  • Hayashi T, Mekata H, Sekiguch S, Kirino Y, Mitoma S, Honkawa K, Horii Y and Norimine J. Cattle with the BoLA class II DRB3*0902 allele have significantly lower bovine leukemia proviral loads. J. Vet. Med. Sci. doi: 10.1292/jvms. 16-0601. 2017
  • Marawan M.A, Mekata H, Hayashi T, Sekiguch S, Kirino Y, Horii Y, Moustafa A.M, Arnaout F.K, Galila E.S and Norimine J. Phylogenetic analysis of env of bovine leukemia virus strains spread in Miyazaki prefecture, Japan. J. Vet. Med. Sci. doi: 10.1292/jvms. 17-0055. 2017
  • Takatsuka K, Sekiguch S, Yamaba H, Kubota S, Okazaki N. Development of a Mathematical Method to Detect Infection on the Farm in the Incubation Period for Foot-and-Mouth Disease. KAGAKU KOGAKU RONBUNSHU. 43:117-122. 2017
  • Yamazaki W, Sabike II, Sekiguch S. High prevalence of Campylobacter in broiler flocks is a crucial factor for food poisoning frequency in humans. Jpn J Infect Dis. Sep 11. doi: 10.7883/yoken.JJID. 2017

2016年

  • Sabike II, Uemura R, Kirino Y, Mekata H, Sekiguch S, Okabayashi T, Goto Y, Yamazaki W. Use of Direct LAMP Screening of Broiler Fecal Samples for Campylobacter jejuni and Campylobacter coli in the Positive Flock Identification Strategy. Front Microbiol. 30;7:1582. eCollection. 2016.
  • Giangaspero M, Sekiguch S. Risk Assessment of Animal Infectious Diseases and Decision Making Process. Clin Microbiol. 5:1-7. 2016
  • Yamazaki W, Uemura R, Sekiguch S, Dong JB, Watanabe S, Kirino Y, Mekata H, Nonaka N, Norimine J, Sueyoshi M, Goto Y, Horii Y, Kurogi M, Yoshino S, Misawa N. Campylobacter and Salmonella are prevalent in broiler farms in Kyushu, Japan: results of a 2-year distribution and circulation dynamics audit. J Appl Microbiol. 120:1711-22. 2016
  • Sasaki Y, Sekiguch S, Uemura R, Sueyoshi M. The effect of depopulation and restocking on reproductive and growth performances on Japanese commercial swine farms. J Vet Med Sci. 78:333-5. 2016
  • Sasaki Y, Alvarez J, Sekiguch S, Sueyoshi M, Otake S, Perez A.Epidemiological factors associated to spread of porcine epidemic diarrhea in Japan. Prev Vet Med. 123:161-7. 2016

2015年

  • Mekata H, Sekiguch S, Konnai S, Kirino Y, Horii Y, Norimine J. Horizontal transmission and phylogenetic analysis of bovine leukemia virus in two districts of Miyazaki, Japan. J Vet Med Sci. 77:1115-20. 2015
  • Mekata H, Sekiguch S, Konnai S, Kirino Y, Honkawa K, Nonaka N, Horii Y and Norimine J. An Evaluation of the Natural Perinatal Transmission of Bovine Leukaemia Virus. Veterinary Record. 7;176:254. 2015

2014年

  • Sasaki Y, Uemura R, Sekiguch S, Takahashi T, Fujii Y and Sueyoshi M. A field survey of factors affecting production performance in broiler flocks in Japanese commercial broiler farms. British Poultry Science. 2014;55:737-44. 2014