VTEC2018

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　　　　VTEC2018_ POSTER (pdf)

　　　　Og-typing primer sequences (Excel)

　　　　hg-typing primer sequences (excel)

References

OG-typing pcr

Iguchi A*, Iyoda S, Seto K, Morita-Ishihara T, Scheutz F, Ohnishi M, and Pathogenic E. coli Working Group in Japan. Escherichia coli O-genotyping PCR; a comprehensive and practical platform for molecular O-serogrouping. Journal of Clinical Microbiology 53(8):2427-32 (2015)

Hg-typing PCR

Banjo M, Iguchi A*, Seto K, Kikuchi T, Harada T, Scheutz F, Iyoda S; Pathogenic E. coli Working Group in Japan. Escherichia coli H-genotyping PCR; a complete and practical platform for molecular H-typing. J Clin Microbiol. 2018 Mar 28. pii: JCM.00190-18. doi: 10.1128/JCM.00190-18.

Reference sequences

Iguchi A*, Iyoda S, Kikuchi T, Ogura Y, Katsura K, Ohnishi M, Hayashi T, Thomson NR. A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster. DNA Research 22(1):101-7 (2015)

Joensen K, Tetzschner A, Iguchi A, Aarestrup F, Scheutz F*. Rapid and easy in silico serotyping of Escherichia coli using Whole genome sequencing (WGS) data. Journal of Clinical Microbiology 53:2410-26 (2015)

Novel og-types

Iguchi A*, Iyoda S, Seto K, Nishii H, Ohnishi M, Mekata H, Ogura Y, Tetsuya H. Six novel O genotypes from Shiga toxin-producing Escherichia coli. Frontiers in Microbiology 20;7:765. (2016)

Iguchi A*, Mentzer von A, Kikuchi T, Thomson R N. An untypeable enterotoxigenic Escherichia coli represents one of the dominant types causing human disease. Microbial Genomics 2017 Jul 3;3(9):e000121.