{"id":34,"date":"2016-01-26T20:01:09","date_gmt":"2016-01-26T11:01:09","guid":{"rendered":"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/?post_type=study-introduction&p=34"},"modified":"2016-01-26T20:01:38","modified_gmt":"2016-01-26T11:01:38","slug":"%e3%82%ab%e3%83%b3%e3%82%ad%e3%83%84%e8%87%aa%e5%ae%b6%e4%b8%8d%e5%92%8c%e5%90%88%e6%80%a7%e3%81%ae%e8%a7%a3%e6%9e%90","status":"publish","type":"study-introduction","link":"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/study-introduction\/34\/","title":{"rendered":"\u30ab\u30f3\u30ad\u30c4\u81ea\u5bb6\u4e0d\u548c\u5408\u6027\u306e\u89e3\u6790"},"content":{"rendered":"

\u3000\u30ab\u30f3\u30ad\u30c4\u985e\u306e\u81ea\u5bb6\u4e0d\u548c\u5408\u6027\u306f\uff0c\u7d4c\u6e08\u683d\u57f9\u3084\u80b2\u7a2e\u306e\u969b\u306e\u91cd\u8981\u306a\u554f\u984c\u3067\u3042\u308b\uff0e\u305d\u306e\u6a5f\u5e8f\u306f\u751f\u6b96\u751f\u7406\u3084\u5206\u5b50\u751f\u7269\u5b66\u7684\u7814\u7a76\u306b\u304a\u3044\u3066\u3044\u304f\u3064\u304b\u306e\u77e5\u898b\u304c\u5f97\u3089\u308c\u3066\u3044\u308b\u3082\u306e\u306e\uff0c\u539f\u56e0\u56e0\u5b50\u306f\u540c\u5b9a\u3055\u308c\u3066\u304a\u3089\u305a\uff0c\u95a2\u9023\u907a\u4f1d\u5b50\u306e\u7ffb\u8a33\u30ec\u30d9\u30eb\u3067\u306e\u7814\u7a76\u306f\u307b\u3068\u3093\u3069\u884c\u308f\u308c\u3066\u3044\u306a\u3044\uff0e\u305d\u3053\u3067\uff0c\u672c\u7814\u7a76\u3067\u306f\uff0c\u30ab\u30f3\u30ad\u30c4\u985e\u306e\u81ea\u5bb6\u4e0d\u548c\u5408\u6027\u306e\u6a5f\u5e8f\u89e3\u660e\u306e\u305f\u3081\u306e\u65b0\u305f\u306a\u77e5\u898b\u3092\u5f97\u308b\u305f\u3081\u306b\uff0c\u95a2\u9023\u56e0\u5b50\u306e\u6709\u52b9\u306a\u540c\u5b9a\u624b\u6cd5\u306b\u3064\u3044\u3066\u691c\u8a0e\u3057\uff0c\u96cc\u96c4\u4e21\u914d\u5076\u5b50\u5074\u304b\u3089\u306e\u30a2\u30d7\u30ed\u30fc\u30c1\u3092\u884c\u3063\u305f\uff0e<\/p>\n

    \n
  1. \u96c4\u6027\u914d\u5076\u5b50\u5074\u306e\u81ea\u5bb6\u4e0d\u548c\u5408\u6027\u306e\u95a2\u9023\u56e0\u5b50\u306e\u63a2\u7d22\u3092\u884c\u3046\u305f\u3081\u306b\uff0c\u30d2\u30e5\u30a6\u30ac\u30ca\u30c4\u82b1\u7c89\u3092\u7528\u3044\u305f\u6db2\u4f53\u82b1\u7c89\u57f9\u990a\u7cfb\u306e\u691c\u8a0e\u3092\u884c\u3044\uff0cin vitro\u306b\u3088\u308b\u81ea\u5bb6\u4e0d\u548c\u5408\u6027\u306e\u73fe\u8c61\u306e\u8996\u899a\u5316\u3084\u5206\u5b50\u751f\u7269\u5b66\u7684\u89e3\u6790\u306e\u6750\u6599\u3092\u4f5c\u88fd\u3059\u308b\u624b\u6cd5\u3092\u78ba\u7acb\u3057\u305f\uff0e\u307e\u305f\uff0c\u30d2\u30e5\u30a6\u30ac\u30ca\u30c4\u666e\u901a\u7cfb\u3068\u81ea\u5bb6\u548c\u5408\u6027\u5909\u7570\u7cfb\u7d71\u306e\u82b1\u7c89\u3068\u96cc\u305a\u3044\u3092\u7528\u3044\u3066\u6db2\u4f53\u82b1\u7c89\u57f9\u990a\u7cfb\u306b\u3088\u308b\u81ea\u5bb6\u4e0d\u548c\u5408\u6027\u306e\u73fe\u8c61\u306e\u89b3\u5bdf\u3092\u884c\u3063\u305f\u3068\u3053\u308d\uff0c\u53d7\u7c89\u8a66\u9a13\u306b\u3088\u308b\u82b1\u7c89\u7ba1\u306e\u6319\u52d5\u3084\u7740\u679c\u8a66\u9a13\u306e\u7d50\u679c\u3068\u4e00\u81f4\u3057\uff0cin vivo\u3067\u306e\u81ea\u5bb6\u4e0d\u548c\u5408\u6027\u73fe\u8c61\u3092in vitro\u3067\u518d\u73fe\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u305f\uff0e\u3055\u3089\u306b\uff0c\u3053\u306e\u6db2\u4f53\u82b1\u7c89\u57f9\u990a\u7cfb\u3092\u7528\u3044\u305f\u95a2\u9023\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u63a2\u7d22\u306e\u305f\u3081\u306b\uff0c\u30d2\u30e5\u30a6\u30ac\u30ca\u30c4\u306e\u96cc\u305a\u3044\u306e\u7c97\u62bd\u51fa\u7269\u51e6\u7406\u3092\u884c\u3063\u305f\u82b1\u7c89\u7ba1\u306e\u30d7\u30ed\u30c6\u30aa\u30fc\u30e0\u89e3\u6790\u3092\u884c\u3063\u305f\uff0e\u305d\u306e\u7d50\u679c\uff0c\u4e0d\u548c\u5408\u53cd\u5fdc\u304c\u751f\u3058\u3066\u3044\u308b\u82b1\u7c89\u7ba1\u306b\u304a\u3044\u3066\uff0c\u30d0\u30e9\u79d1\u306e\u81ea\u5bb6\u4e0d\u548c\u5408\u6027\u306e\u96c4\u6027\u914d\u5076\u5b50\u5074\u56e0\u5b50\u3067\u3042\u308bF-box \u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u91cf\u304c\u4f4e\u4e0b\u3059\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u3063\u305f\uff0e\u305d\u306e\u6a5f\u5e8f\u306f\u4e0d\u660e\u3067\u3042\u308b\u304c\uff0c\u540c\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u6e1b\u5c11\u306f\u81ea\u5bb6\u4e0d\u548c\u5408\u6027\u53cd\u5fdc\u3092\u5f15\u304d\u8d77\u3053\u3057\u3066\u3044\u308b\u53ef\u80fd\u6027\u304c\u793a\u5506\u3055\u308c\u305f\uff0e\u4ee5\u4e0a\u306e\u3088\u3046\u306b\uff0c\u30ab\u30f3\u30ad\u30c4\u985e\u306b\u304a\u3051\u308b\u81ea\u5bb6\u4e0d\u548c\u5408\u6027\u306e\u95a2\u9023\u56e0\u5b50\u306e\u63a2\u7d22\u306b\u306f\uff0c\u30d7\u30ed\u30c6\u30aa\u30fc\u30e0\u89e3\u6790\u3068\u6db2\u4f53\u82b1\u7c89\u57f9\u990a\u304c\u6709\u52b9\u3067\u3042\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u305f\uff0e\u4eca\u5f8c\uff0c\u672c\u6280\u8853\u3092\u5229\u7528\u3057\u305f\u96c4\u6027\u5074\u95a2\u9023\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u540c\u5b9a\u3084\u85ac\u5264\u5fdc\u7b54\u306e\u89e3\u6790\u3092\u884c\u3046\u3053\u3068\u306b\u3088\u308a\uff0c\u81ea\u5bb6\u4e0d\u548c\u5408\u6027\u306e\u6a5f\u5e8f\u89e3\u660e\u3084\u548c\u5408\u6027\u8a98\u5c0e\u306b\u3088\u308b\u7740\u679c\u4fc3\u9032\u5264\u306e\u958b\u767a\u3078\u306e\u5fdc\u7528\u304c\u671f\u5f85\u3055\u308c\u308b\uff0e\uff08Uchida et al. 2012a, 2012b\uff09\n
    \n\"\u6db2\u4f53\u82b1\u7c89\u57f9\u990a\u306b\u3088\u308a\u6b63\u5e38\u306b\u767a\u82bd\u3057\u305f\u82b1\u7c89\u3068\u7834\u88c2\u3057\u305f\u82b1\"
    \n\u56f3\u3000\u6db2\u4f53\u82b1\u7c89\u57f9\u990a\u306b\u3088\u308a\u6b63\u5e38\u306b\u767a\u82bd\u3057\u305f\u82b1\u7c89\u3068\u7834\u88c2\u3057\u305f\u82b1\u7c89<\/span>\n<\/div>\n
    \n\"\u56f3\u3000\u548c\u5408\uff0f\u4e0d\u548c\u5408\u73fe\u8c61\u3092\u793a\u3057\u305f\u82b1\u7c89\u7ba1\u3092\u5229\u7528\u3057\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u540c\u5b9a\"
    \n\u56f3\u3000\u548c\u5408\uff0f\u4e0d\u548c\u5408\u73fe\u8c61\u3092\u793a\u3057\u305f\u82b1\u7c89\u7ba1\u3092\u5229\u7528\u3057\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u540c\u5b9a<\/span>\n<\/div>\n<\/li>\n
  2. \u30dd\u30ea\u30a2\u30df\u30f3\u306f\u3001\u4f4e\u5206\u5b50\u306e\u8102\u80aa\u65cf\u30dd\u30ea\u30ab\u30c1\u30aa\u30f3\u3067\u3042\u308a\u3001\u69d8\u3005\u306a\u7d30\u80de\u751f\u7406\u6a5f\u80fd\u306b\u5e83\u304f\u95a2\u308f\u3063\u3066\u304a\u308a\u3001\u30dd\u30ea\u30a2\u30df\u30f3\u5408\u6210\u95a2\u9023\u907a\u4f1d\u5b50\u306e\u6a5f\u80fd\u306e\u6b20\u5982\u3084\u904e\u5270\u767a\u73fe\u306f\u82b1\u7c89\u767a\u82bd\u3084\u82b1\u7c89\u7ba1\u306e\u4f38\u9577\u3092\u6291\u5236\u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u308b\u3002\u305d\u3053\u3067\u3001\u672c\u7814\u7a76\u3067\u306f\u30ab\u30f3\u30ad\u30c4\u306e\u81ea\u5bb6\u4e0d\u548c\u5408\u6027\u3068\u30dd\u30ea\u30a2\u30df\u30f3\u3068\u306e\u95a2\u4fc2\u3092\u89e3\u660e\u3059\u308b\u305f\u3081\u306b\u3001\u5f37\u3044\u81ea\u5bb6\u4e0d\u548c\u5408\u6027\u3092\u6709\u3059\u308b’\u6669\u767d\u67da’\u3068’\u65e5\u5411\u590f’\u306e\u767a\u82bd\u3057\u305f\u82b1\u7c89\u7ba1\u306b\u548c\u5408\u304a\u3088\u3073\u81ea\u5bb6\u4e0d\u548c\u5408\u69d8\u51e6\u7406\u3092\u884c\u3044\u3001\u82b1\u7c89\u7ba1\u5185\u90e8\u306e\u30dd\u30ea\u30a2\u30df\u30f3\u542b\u91cf\u3092\u5206\u6790\u3057\u305f\u3002\u307e\u305f\u3001\u548c\u5408\u304a\u3088\u3073\u81ea\u5bb6\u4e0d\u548c\u5408\u69d8\u51e6\u7406\u3057\u305f\u82b1\u7c89\u7ba1\u306b\u304a\u3044\u3066nano-LC-MS\u3067\u5206\u6790\u3092\u884c\u3044\u3001\u540c\u5b9a\u3057\u305f\u81ea\u5bb6\u4e0d\u548c\u5408\u95a2\u9023\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u3068\u30dd\u30ea\u30a2\u30df\u30f3\u3068\u306e\u95a2\u4fc2\u306b\u3064\u3044\u3066\u8003\u5bdf\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3001\u30dd\u30ea\u30a2\u30df\u30f3\u306e\u3072\u3068\u3064\u3067\u3042\u308b\u30b9\u30da\u30eb\u30df\u30b8\u30f3\u3092\u5916\u751f\u51e6\u7406\u3057\u305f\u82b1\u7c89\u7ba1\u306f\u3001\u81ea\u5bb6\u4e0d\u548c\u5408\u69d8\u53cd\u5fdc\u3068\u540c\u69d8\u306e\u7570\u5e38\u306a\u5f62\u614b\u304c\u89b3\u5bdf\u3055\u308c\u3001\u82b1\u7c89\u7ba1\u5185\u306e\u30dd\u30ea\u30a2\u30df\u30f3\u306e\u542b\u91cf\u3082\u5897\u52a0\u3057\u305f\u3002\u307e\u305f\u3001\u5019\u88dc\u907a\u4f1d\u5b50\u3068\u3057\u3066\u540c\u5b9a\u3055\u308c\u305f\u3001Cu\/Zn superoxide dismutase\u3001Mn superoxide dismutase\u3001catalase \u304a\u3088\u3073cysteine protease\u306e\u767a\u73fe\u304c\u81ea\u5bb6\u4e0d\u548c\u5408\u69d8\u51e6\u7406\u306b\u3088\u308a\u5897\u52a0\u3057\u3001\u5916\u751f\u7684\u306a\u30b9\u30da\u30eb\u30df\u30b8\u30f3\u51e6\u7406\u306f\u81ea\u5bb6\u4e0d\u548c\u5408\u69d8\u51e6\u7406\u3068\u307b\u307c\u540c\u69d8\u306e\u767a\u73fe\u50be\u5411\u304c\u78ba\u8a8d\u3055\u308c\u305f\u3002\u307e\u305f\u3001\u540c\u51e6\u7406\u306f\u82b1\u7c89\u7ba1\u306e\u6d3b\u6027\u9178\u7d20\uff08ROS\uff09\u3068\u30ab\u30eb\u30b7\u30a6\u30e0\u542b\u91cf\u306e\u5897\u52a0\u304a\u3088\u3073\u30a2\u30af\u30c1\u30f3\u7e4a\u7dad\u306e\u8131\u91cd\u5408\u306e\u4fc3\u9032\u304c\u78ba\u8a8d\u3055\u308c\u305f\u3002\u4ee5\u4e0a\u306e\u3088\u3046\u306b\u3001\u30dd\u30ea\u30a2\u30df\u30f3\u304c\u30ab\u30f3\u30ad\u30c4\u82b1\u7c89\u7ba1\u306e\u81ea\u5bb6\u4e0d\u548c\u5408\u69d8\u53cd\u5fdc\u3092\u8a98\u767a\u3067\u304d\u308b\u3070\u304b\u308a\u3067\u306a\u304f\u3001\u81ea\u5bb6\u4e0d\u548c\u5408\u6027\u95a2\u9023\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u91cf\u3092\u5897\u52a0\u3059\u308b\u3053\u3068\u3092\u660e\u3089\u304b\u306b\u3057\u305f\u3002\u307e\u305f\u3001\u3053\u308c\u3089\u306e\u82b1\u7c89\u7ba1\u306e\u4e00\u9023\u306e\u5909\u5316\u306f\u3001\u4e00\u7a2e\u306e\u30d7\u30ed\u30b0\u30e9\u30e0\u7d30\u80de\u6b7b\u306e\u73fe\u8c61\u3068\u4e00\u81f4\u3057\u3066\u3044\u305f\u3002\u82b1\u7c89\u7ba1\u5185\u306e\u30dd\u30ea\u30a2\u30df\u30f3\u91cf\u306e\u5909\u5316\u306f\u81ea\u5bb6\u4e0d\u548c\u5408\u6027\u3092\u5236\u5fa1\u3059\u308b\u91cd\u8981\u306a\u8981\u56e0\u304b\u3082\u3057\u308c\u306a\u3044\u3002\uff08Li et al., 2015a, 2015b\uff09\n
    \n\"\u56f3\u3000\u6db2\u4f53\u82b1\u7c89\u57f9\u990a\u306b\u304a\u3044\u3066\u548c\u5408\uff0f\u4e0d\u548c\u5408\u69d8\u73fe\u8c61\u3067\u767a\u73fe\u3057\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u985e\"
    \n\u56f3\u3000\u6db2\u4f53\u82b1\u7c89\u57f9\u990a\u306b\u304a\u3044\u3066\u548c\u5408\uff0f\u4e0d\u548c\u5408\u69d8\u73fe\u8c61\u3067\u767a\u73fe\u3057\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u985e<\/span>\n<\/div>\n
    \n\"\u56f3\u3000\u81ea\u5bb6\u4e0d\u548c\u5408\u95a2\u9023\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u89e3\u6790\"
    \n\u56f3\u3000\u81ea\u5bb6\u4e0d\u548c\u5408\u95a2\u9023\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u89e3\u6790<\/span>\n<\/div>\n<\/li>\n<\/ol>\n
      \n
    1. 1\uff09Uchida, U., H. Sassa, S. Takenaka, Y. Sakakibara, M. Suiko, H. Kunitake, Identification of self-incompatibility related proteins in the pistil of Japanese pear (Pyrus pyrifolia (Burm.f.)) by proteome analysis. Plant Omics Journal, 5(4):320-325, 2012a <\/li>\n
    2. 2\uff09Uchida, A., S. Takenaka, Y. Sakakibara, S. Kurogi,, C. Honsho, H. Sassa, M. Suiko and H. Kunitake, Comprehensive Analysis of Expressed Proteins in the Different Stages of the Style Development of Self-incompatible ‘Hyuganatsu’ (Citrus tamurana hort. Ex Tanaka)\u00a0 J Japan Soc Hort Sci. 81:150-158, 2012b<\/li>\n
    3. 3\uff09Honsho, C., E.Yamamura, K. Tsuruta, Y. Yoshimaru, K. Yasuda, A. Uchida, H. Kunitake and T. Tetsumura. Unreduced 2n pollen production in ‘Nishiuchi Konatsu’\u00a0 Hyuganatsu as inferred by pollen characteristics and progeny ploidy level.\u00a0 J Japan Soc Hort Sci., 81:19-26, 2012<\/li>\n
    4. 4\uff09\u5185\u7530\u98db\u9999\u30fb\u5b89\u90e8\u79cb\u6674\u30fb\u661f\u91ce\u6d0b\u4e00\u90ce\u30fb\u570b\u6b66\u4e45\u767b. \u30d2\u30e5\u30a6\u30ac\u30ca\u30c4\uff08Citrus tamurana hort. ex Tanaka\uff09\u306b\u304a\u3051\u308b\u6210\u719f\u82b1\u7c89\u306e\u6db2\u4f53\u57f9\u990a\u7cfb\u306e\u78ba\u7acb. \u5712\u82b8\u5b66\u7814\u7a76,\u300011:173-179,2012<\/li>\n
    5. 5\uff09Li, Y., Abe, A., T. Fuse, T. Hirano, Y. Hoshino, H. Kunitake, In vitro self-incompatible-like response applied for protein identification and gene expression analysis in Citrus cultivars, Banpeiyu and Hyuganatsu. J Amer Soc for Hortic Sci. 140(4):339\u2013345, 2015a<\/li>\n
    6. 6\uff09Y. Li, A. Uchida, A. Abe, A. Yamamoto, T. Hirano, H. Kunitake Effect of polyamine on self-incompatibility-like responses in pollen tubes of Citrus cultivars, Banpeiyu and Hyuganatsu. Journal of American Society of Horticultural Sciences, 140 (2): 183\u2013190, 2015b\u3000<\/li>\n<\/ol>\n","protected":false},"template":"","study-intro":[2],"class_list":["post-34","study-introduction","type-study-introduction","status-publish","hentry","study-intro-fruiter"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/wp-json\/wp\/v2\/study-introduction\/34","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/wp-json\/wp\/v2\/study-introduction"}],"about":[{"href":"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/wp-json\/wp\/v2\/types\/study-introduction"}],"wp:attachment":[{"href":"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/wp-json\/wp\/v2\/media?parent=34"}],"wp:term":[{"taxonomy":"study-intro","embeddable":true,"href":"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/wp-json\/wp\/v2\/study-intro?post=34"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}