{"id":61,"date":"2016-01-26T18:34:46","date_gmt":"2016-01-26T09:34:46","guid":{"rendered":"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/?post_type=study-introduction&#038;p=61"},"modified":"2016-02-02T12:02:44","modified_gmt":"2016-02-02T03:02:44","slug":"%e3%82%ad%e3%82%a4%e3%83%81%e3%82%b4%e3%81%ae%e5%9c%a8%e6%9d%a5%e7%a8%ae%e3%82%92%e5%88%a9%e7%94%a8%e3%81%97%e3%81%9f%e8%82%b2%e7%a8%ae","status":"publish","type":"study-introduction","link":"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/study-introduction\/61\/","title":{"rendered":"\u30ad\u30a4\u30c1\u30b4\u306e\u5728\u6765\u7a2e\u3092\u5229\u7528\u3057\u305f\u80b2\u7a2e"},"content":{"rendered":"<p>\u30e9\u30ba\u30d9\u30ea\u30fc\u3084\u30d6\u30e9\u30c3\u30af\u30d9\u30ea\u30fc\u306a\u3069\u306e\u30ad\u30a4\u30c1\u30b4\u306f\u751f\u98df\u3060\u3051\u3067\u306a\u304f\u30b8\u30e3\u30e0\u306a\u3069\u306e\u52a0\u5de5\u98df\u54c1\u3068\u3057\u3066\u3082\u5229\u7528\u4fa1\u5024\u304c\u9ad8\u3044\u5c0f\u679c\u6a39\u3067\u3042\u308b\uff0e\u65e5\u672c\u306b\u306f40\u7a2e\u4ee5\u4e0a\u306e\u91ce\u751f\u7a2e\u304c\u81ea\u751f\u3057\u3066\u3044\u308b\u304c\uff0c\u305d\u308c\u3089\u306e\u5065\u5eb7\u6a5f\u80fd\u6027\u3092\u542b\u3081\u305f\u679c\u5b9f\u54c1\u8cea\u306e\u8a55\u4fa1\u304a\u3088\u3073\u80b2\u7a2e\u7684\u5229\u7528\u306f\u307b\u3068\u3093\u3069\u306a\u3055\u308c\u3066\u3044\u306a\u3044\uff0e\u305d\u3053\u3067\uff0c\u672c\u7814\u7a76\u3067\u306f\uff0c\u30ad\u30a4\u30c1\u30b4\u683d\u57f9\u54c1\u7a2e\u304a\u3088\u3073\u6696\u5730\u6027\u91ce\u751f\u7a2e\u306b\u3064\u3044\u3066\uff0c\u3044\u304f\u3064\u304b\u306e\u6a5f\u80fd\u6027\u3092\u6307\u6a19\u3068\u3057\u3066\u80b2\u7a2e\u7d20\u6750\u306e\u9078\u629c\u3092\u884c\u3044\uff0c\u30e9\u30ba\u30d9\u30ea\u30fc\u3068\u30ca\u30ef\u30b7\u30ed\u30a4\u30c1\u30b4\u3068\u306e\u7a2e\u9593\u96d1\u7a2e\u3092\u80b2\u6210\u3057\u305f\uff0e<\/p>\n<ol>\n<li>\u690d\u7269\u6750\u6599\u306b\u306f\uff0c\u30e9\u30ba\u30d9\u30ea\u30fc2\u54c1\u7a2e\uff0c\u30d6\u30e9\u30c3\u30af\u30d9\u30ea\u30fc2\u54c1\u7a2e\u304a\u3088\u3073\u6696\u5730\u6027\u91ce\u751f\u7a2e5\u7a2e\u3092\u4f9b\u8a66\u3057\u305f\uff0e\u307e\u305a\uff0c\u7dcf\u30dd\u30ea\u30d5\u30a7\u30ce\u30fc\u30eb\u542b\u91cf\uff0c\u6297\u9178\u5316\u80fd\u304a\u3088\u3073\u30d2\u30c8\u524d\u9aa8\u9ac4\u6027\u767d\u8840\u75c5\u7d30\u80de(HL-60)\u306e\u5897\u6b96\u6291\u5236\u306b\u3064\u3044\u3066\u8a55\u4fa1\u3057\u305f\u3068\u3053\u308d\uff0c\u30d6\u30e9\u30c3\u30af\u30d9\u30ea\u30fc\u2018Martonthornless\u2019\uff0c\u30ca\u30ac\u30d0\u30e2\u30df\u30b8\u30a4\u30c1\u30b4\u304a\u3088\u3073\u30ca\u30ef\u30b7\u30ed\u30a4\u30c1\u30b4\u306e3\u7a2e\u304c\u9ad8\u3044\u5024\u3092\u793a\u3057\u305f\uff0e\u307e\u305f\uff0c\u305d\u308c\u3089\u306e\u8a55\u4fa1\u9805\u76ee\u306b\u95a2\u3057\u3066\u9ad8\u3044\u76f8\u95a2\u304c\u78ba\u8a8d\u3055\u308c\uff0c\u30dd\u30ea\u30d5\u30a7\u30ce\u30fc\u30eb\u304c\u6297\u9178\u5316\u80fd\u304a\u3088\u3073HL-60\u7d30\u80de\u5897\u6b96\u6291\u5236\u306b\u5f71\u97ff\u3057\u3066\u3044\u308b\u53ef\u80fd\u6027\u304c\u793a\u5506\u3055\u308c\u305f\uff0e\u6b21\u306b\uff0c\u9078\u629c\u3057\u305f3\u7a2e\u306b\u3064\u3044\u3066HL-60\u7d30\u80de\u5897\u6b96\u6291\u5236\u306b\u5bc4\u4e0e\u3059\u308b\u6d3b\u6027\u6210\u5206\u3092\u540c\u5b9a\u3059\u308b\u305f\u3081\u306b\uff0cHPLC\u304a\u3088\u3073LC-MS\u5206\u6790\u306b\u3088\u308b\u679c\u5b9f\u62bd\u51fa\u7269\u4e2d\u306e\u4e3b\u8981\u306a\u30dd\u30ea\u30d5\u30a7\u30ce\u30fc\u30eb\u306e\u5b9a\u6027\u304a\u3088\u3073\u5b9a\u91cf\uff0c\u3055\u3089\u306bPorter\u6cd5\u306b\u3088\u308b\u30d7\u30ed\u30a2\u30f3\u30c8\u30b7\u30a2\u30cb\u30b8\u30f3\u306e\u5b9a\u91cf\u3092\u884c\u3063\u305f\uff0e\u305d\u306e\u7d50\u679c\uff0c\u30a8\u30e9\u30b8\u30bf\u30f3\u30cb\u30f3\u3068\u30d7\u30ed\u30a2\u30f3\u30c8\u30b7\u30a2\u30cb\u30b8\u30f3\u304c\u4e3b\u8981\u306a\u30dd\u30ea\u30d5\u30a7\u30ce\u30fc\u30eb\u3067\u3042\u308a\uff0c\u305d\u306e\u542b\u91cf\u306b\u306f\u6750\u6599\u306b\u3088\u3063\u3066\u5dee\u7570\u304c\u89b3\u5bdf\u3055\u308c\u305f\uff0e\u307e\u305f\uff0c\u305d\u308c\u3089\u306e\u62bd\u51fa\u753b\u5206\u306b\u304a\u3051\u308bHL-60\u7d30\u80de\u5897\u6b96\u6291\u5236\u8a66\u9a13\u306e\u7d50\u679c\uff0c\u305d\u308c\u3089\u306e\u30dd\u30ea\u30d5\u30a7\u30ce\u30fc\u30eb\u304c\u7d30\u80de\u5897\u6b96\u6291\u5236\u306b\u5927\u304d\u304f\u95a2\u4e0e\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u3068\u306a\u3063\u305f\uff0e\u307e\u305f\uff0c\u305d\u308c\u3089\u306e\u5bc4\u4e0e\u7387\u306f\u6750\u6599\u306b\u3088\u3063\u3066\u5dee\u7570\u304c\u89b3\u5bdf\u3055\u308c\uff0c\u30ca\u30ef\u30b7\u30ed\u30a4\u30c1\u30b4\u3067\u306f\u30a8\u30e9\u30b8\u30bf\u30f3\u30cb\u30f3\u304c\u6700\u3082\u5927\u304d\u304f\u5f71\u97ff\u3057\u3066\u3044\u308b\u53ef\u80fd\u6027\u304c\u793a\u5506\u3055\u308c\u305f\uff0e\n<div class=\"center-box\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kiichigo01.jpg\" alt=\"HL-60\u7d30\u80de\u306e\u5f62\u614b\u5909\u5316\" width=\"342\" height=\"423\" class=\"alignnone size-full wp-image-62\" srcset=\"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kiichigo01.jpg 342w, https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kiichigo01-243x300.jpg 243w\" sizes=\"auto, (max-width: 342px) 85vw, 342px\" \/><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kiichigo02.jpg\" alt=\"DNA\u30e9\u30c0\u30fc\u306e\u691c\u51fa\" width=\"292\" height=\"423\" class=\"alignnone size-full wp-image-63\" srcset=\"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kiichigo02.jpg 292w, https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kiichigo02-207x300.jpg 207w\" sizes=\"auto, (max-width: 292px) 85vw, 292px\" \/><br \/>\n<span class=\"img-name\">\uff08\u7d30\u80de\u5bc6\u5ea6\uff1a1.0\u00d7105cells\/ml, \u30b5\u30f3\u30d7\u30eb\u6fc3\u5ea6\uff1a2mg\/ml, 3\u6642\u9593\u51e6\u7406\uff09<\/span>\n<\/div>\n<\/li>\n<li>\u9078\u629c\u3055\u308c\u305f\u6696\u5730\u6027\u91ce\u751f\u7a2e\u30ca\u30ef\u30b7\u30ed\u30a4\u30c1\u30b4\u3068\u30e9\u30ba\u30d9\u30ea\u30fc\u683d\u57f9\u54c1\u7a2e\u3068\u306e\u7a2e\u9593\u4ea4\u96d1\u3092\u884c\u3063\u305f\uff0e\u5f97\u3089\u308c\u305f\u7a2e\u9593\u96d1\u7a2e\u306e\u307b\u3068\u3093\u3069\u306f\uff0c\u30ca\u30ef\u30b7\u30ed\u30a4\u30c1\u30b4\u306e\u307b\u3075\u304f\u6027\u306e\u6a39\u59ff\u3092\u793a\u3057\uff0c\u6a39\u52e2\u304c\u5f37\u304f\uff0c\u30e9\u30ba\u30d9\u30ea\u30fc\u3068\u540c\u7a0b\u5ea6\u306e\u679c\u5b9f\u306e\u5927\u304d\u3055\u3092\u6709\u3059\u308b\u3082\u306e\u3082\u3042\u3063\u305f\uff0e\u7279\u306b\uff0cIP-1\u7cfb\u7d71\u306f\u30e9\u30ba\u30d9\u30ea\u30fc\u3088\u308a\u3082\u679c\u5b9f\u304c\u6709\u610f\u306b\u5927\u304d\u304f\uff0c\u98df\u5473\u304c\u826f\u3044\u3068\u3044\u3046\u7279\u5fb4\u3092\u6709\u3057\uff0c\u30a8\u30e9\u30b8\u30bf\u30f3\u30cb\u30f3\u542b\u91cf\u306f\u30e9\u30ba\u30d9\u30ea\u30fc\u306e\u7d044\u500d\u3068\u306a\u3063\u3066\u3044\u305f\uff0e\n<div class=\"center-box\" style=\"margin-bottom:0;\">\n<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kiichigo03.jpg\" alt=\"\u8449\u3000\u679c\u5b9f\" width=\"311\" height=\"366\" class=\"margin-bottom alignnone size-full wp-image-64\" srcset=\"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kiichigo03.jpg 311w, https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kiichigo03-255x300.jpg 255w\" sizes=\"auto, (max-width: 311px) 85vw, 311px\" \/><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kiichigo04.jpg\" alt=\"\u82b1\u3000\u82b1\u7c89\" width=\"312\" height=\"366\" class=\"margin-bottom alignnone size-full wp-image-65\" srcset=\"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kiichigo04.jpg 312w, https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kiichigo04-256x300.jpg 256w\" sizes=\"auto, (max-width: 312px) 85vw, 312px\" \/>\n<\/div>\n<div class=\"center-box\" style=\"margin-top:0;\">\n<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kiichigo05.jpg\" alt=\"\u56f3\u3000\u6696\u5730\u6027\u91ce\u751f\u7a2e\u30ca\u30ef\u30b7\u30ed\u30a4\u30c1\u30b4\u3068\u30e9\u30ba\u30d9\u30ea\u30fc\u683d\u57f9\u54c1\u7a2e\u3068\u306e\u7a2e\u9593\u96d1\u7a2e\" width=\"645\" height=\"429\" class=\"alignnone size-full wp-image-66\" srcset=\"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kiichigo05.jpg 645w, https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kiichigo05-300x200.jpg 300w\" sizes=\"auto, (max-width: 709px) 85vw, (max-width: 909px) 67vw, (max-width: 984px) 61vw, (max-width: 1362px) 45vw, 600px\" \/><br \/>\n<span class=\"img-name\">\u56f3\u3000\u6696\u5730\u6027\u91ce\u751f\u7a2e\u30ca\u30ef\u30b7\u30ed\u30a4\u30c1\u30b4\u3068\u30e9\u30ba\u30d9\u30ea\u30fc\u683d\u57f9\u54c1\u7a2e\u3068\u306e\u7a2e\u9593\u96d1\u7a2e<\/span>\n<\/div>\n<\/li>\n<li>\u9ed2\u30e9\u30ba\u30d9\u30ea\u30fc\uff08Rubus 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