{"id":86,"date":"2016-01-26T19:54:19","date_gmt":"2016-01-26T10:54:19","guid":{"rendered":"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/?post_type=study-introduction&#038;p=86"},"modified":"2016-01-26T20:00:16","modified_gmt":"2016-01-26T11:00:16","slug":"%e3%82%ad%e3%83%b3%e3%82%ab%e3%83%b3%e5%b1%9e%e6%a4%8d%e7%89%a9%e3%81%ae%e7%b3%bb%e7%b5%b1%e5%88%86%e9%a1%9e","status":"publish","type":"study-introduction","link":"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/study-introduction\/86\/","title":{"rendered":"\u30ad\u30f3\u30ab\u30f3\u5c5e\u690d\u7269\u306e\u7cfb\u7d71\u5206\u985e"},"content":{"rendered":"<p>\u30ad\u30f3\u30ab\u30f3\u5c5e\u690d\u7269\u306f\uff0c\u679c\u76ae\u90e8\u304c\u751f\u98df\u3067\u304d\u308b\u5c0f\u3055\u306a\u679c\u5b9f\u3092\u6709\u3059\u308b\u30ab\u30f3\u30ad\u30c4\u985e\u3067\u3042\u308a\uff0c\u73fe\u5728\u65e5\u672c\u3067\u306e\u9700\u8981\u306f\u5e83\u304c\u308a\u3064\u3064\u3042\u308b\u304c\uff0c\u305d\u306e\u5b66\u8853\u7684\u306a\u7814\u7a76\u306f\u307b\u3068\u3093\u3069\u884c\u308f\u308c\u3066\u3044\u306a\u3044\uff0e\u305d\u306e\u305f\u3081\uff0c\u30ad\u30f3\u30ab\u30f3\u5c5e\u306e\u5206\u985e\u306f\u672a\u3060\u6df7\u6c8c\u3068\u3057\u3066\u304a\u308a\uff0c\u307e\u305f\uff0c\u80b2\u7a2e\u3082\u9032\u3093\u3067\u304a\u3089\u305a\uff0c\u30ad\u30f3\u30ab\u30f3\u54c1\u7a2e\u306e\u591a\u69d8\u6027\u306f\u6975\u3081\u3066\u4e4f\u3057\u3044\u306e\u304c\u73fe\u72b6\u3067\u3042\u308b\uff0e\u305d\u3053\u3067\u672c\u7814\u7a76\u3067\u306f\uff0c\u30ad\u30f3\u30ab\u30f3\u5c5e\u690d\u7269\u306b\u304a\u3051\u308b\u7cfb\u7d71\u5206\u985e\u3068\u80b2\u7a2e\u306e\u305f\u3081\u306e\u57fa\u790e\u7684\u77e5\u898b\u3092\u5f97\u308b\u305f\u3081\u306b\u4ee5\u4e0b\u306e\u5b9f\u9a13\u3092\u884c\u3063\u305f\uff0e<\/p>\n<ol>\n<li>\u30ad\u30f3\u30ab\u30f3\u5c5e\u690d\u7269\u306b\u304a\u3051\u308b\u7d30\u80de\u907a\u4f1d\u5b66\u7684\u304a\u3088\u3073\u9032\u5316\u5b66\u7684\u306a\u7cfb\u7d71\u5206\u985e\u306e\u77e5\u898b\u3092\u5f97\u308b\u305f\u3081\u306b\uff0c\u30d5\u30ed\u30fc\u30b5\u30a4\u30c8\u30e1\u30c8\u30ea\u30fc\u306b\u3088\u308b\u30b2\u30ce\u30e0\u30b5\u30a4\u30ba\u5206\u6790\uff0c CMA\u6838\u578b\u5206\u6790\u304a\u3088\u3073RAPD\u3068CAPS\u30de\u30fc\u30ab\u30fc\u3092\u7528\u3044\u305fDNA\u591a\u578b\u89e3\u6790\u306b\u57fa\u3065\u3044\u305f\u7dcf\u5408\u7684\u306a\u8a55\u4fa1\u3092\u884c\u3063\u305f\uff0e\u305d\u306e\u7d50\u679c\uff0c\u30ad\u30f3\u30ab\u30f3\u5c5e\u306e\u7a2e\u3068\u3057\u3066\u306f\uff0c\u30de\u30e1\u30ad\u30f3\u30ab\u30f3\u3068\u30ca\u30ac\u30ad\u30f3\u30ab\u30f3\u30b3\u30f3\u30d7\u30ec\u30c3\u30af\u30b9\uff08\u30ca\u30ac\u30ad\u30f3\u30ab\u30f3\uff0c\u30de\u30eb\u30ad\u30f3\u30ab\u30f3\u304a\u3088\u3073\u30cb\u30f3\u30dd\u30a6\u30ad\u30f3\u30ab\u30f3\uff09\u306e2\u7a2e\u306e\u307f\u3067\u3042\u308a\uff0c\u30ca\u30ac\u30cf\u30ad\u30f3\u30ab\u30f3\u3068\u30c1\u30e7\u30a6\u30b8\u30e5\u30ad\u30f3\u30ab\u30f3\u306f\uff0c\u96d1\u7a2e\u3068\u3057\u3066\u5206\u985e\u3055\u308c\u308b\u3079\u304d\u3067\u3042\u308b\u3053\u3068\u3092\u7d50\u8ad6\u4ed8\u3051\u305f\uff0e\uff08Yasuda et al., 2016\uff09\n<div class=\"center-box\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-88\" src=\"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kinkan01.jpg\" alt=\"\u56f3\u3000\u30ad\u30f3\u30ab\u30f3\u5c5e\u690d\u7269\u306e\u67d3\u8272\u4f53\u306eCMA\u67d3\u8272\u50cf\" width=\"455\" height=\"443\" srcset=\"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kinkan01.jpg 455w, https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kinkan01-300x292.jpg 300w\" sizes=\"auto, (max-width: 455px) 85vw, 455px\" \/> <span class=\"img-name\">\u56f3\u3000\u30ad\u30f3\u30ab\u30f3\u5c5e\u690d\u7269\u306e\u67d3\u8272\u4f53\u306eCMA\u67d3\u8272\u50cf<\/span><\/div>\n<\/li>\n<li>\u30ad\u30f3\u30ab\u30f3\u5c5e\u690d\u72696\u7a2e\u3092\u54c1\u7a2e\u80b2\u6210\u306e\u305f\u3081\u306e\u907a\u4f1d\u8cc7\u6e90\u3068\u3057\u3066\u8a55\u4fa1\u3059\u308b\u305f\u3081\u306b\uff0c\u8449\uff0c\u82b1\u304a\u3088\u3073\u679c\u5b9f\u306e\u5f62\u614b\u5b66\u7684\u7279\u5fb4\u3068\u679c\u5b9f\u306b\u304a\u3051\u308b\u7cd6\u30fb\u6709\u6a5f\u9178\u542b\u91cf\uff0c\u03b2-\u30af\u30ea\u30d7\u30c8\u30ad\u30b5\u30f3\u30c1\u30f3\u542b\u91cf\u304a\u3088\u3073DPPH\u30e9\u30b8\u30ab\u30eb\u6d88\u53bb\u80fd\u306b\u3064\u3044\u3066\u8abf\u67fb\u3057\u305f\u3068\u3053\u308d\uff0c\u30de\u30e1\u30ad\u30f3\u30ab\u30f3\u306b\u304a\u3051\u308b\u9ad8\u3044\u03b2-\u30af\u30ea\u30d7\u30c8\u30ad\u30b5\u30f3\u30c1\u30f3\u542b\u91cf\u3084DPPH\u30e9\u30b8\u30ab\u30eb\u6d88\u53bb\u80fd\uff0c\u30c1\u30e7\u30a6\u30b8\u30e5\u30ad\u30f3\u30ab\u30f3\u679c\u76ae\u306e\u9ad8\u3044\u7cd6\u542b\u91cf\u7b49\u304c\u660e\u3089\u304b\u3068\u306a\u308a\uff0c\u30ad\u30f3\u30ab\u30f3\u5c5e\u91ce\u751f\u7a2e\u304c\uff0c\u7279\u5fb4\u7684\u306a\u54c1\u7a2e\u3092\u80b2\u6210\u3059\u308b\u4e0a\u3067\uff0c\u8cb4\u91cd\u306a\u5f62\u8cea\u3092\u6709\u3059\u308b\u3053\u3068\u3092\u793a\u5506\u3057\u305f\uff0e\uff08Yasuda et al. 2010a\uff09\n<div class=\"center-box\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-89 alignnone\" src=\"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kinkan02.jpg\" alt=\"\u56f3\u3000\u30ad\u30f3\u30ab\u30f3\u5c5e\u690d\u7269\u306e\u679c\u5b9f\u5f62\u614b A\uff1a\u30ca\u30ac\u30d0\u30ad\u30f3\u30ab\u30f3\u3001B\uff1a\u30de\u30eb\u30ad\u30f3\u30ab\u30f3\u3001C\uff1a\u30de\u30e1\u30ad\u30f3\u30ab\u30f3\u3001D\uff1a\u30cb\u30f3\u30dd\u30a6\u30ad\u30f3\u30ab\u30f3\u3001E\uff1a\u30ca\u30ac\u30df\u30ad\u30f3\u30ab\u30f3\u3001F\uff1a\u30d5\u30af\u30b7\u30e5\u30a6\u30ad\u30f3\u30ab\u30f3\" width=\"455\" height=\"569\" srcset=\"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kinkan02.jpg 455w, https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kinkan02-240x300.jpg 240w\" sizes=\"auto, (max-width: 455px) 85vw, 455px\" \/> <\/div>\n<p><span class=\"img-name\" style=\"margin: -25px 0 25px\">\u56f3\u3000\u30ad\u30f3\u30ab\u30f3\u5c5e\u690d\u7269\u306e\u679c\u5b9f\u5f62\u614b<br \/>\n A\uff1a\u30ca\u30ac\u30d0\u30ad\u30f3\u30ab\u30f3\u3001B\uff1a\u30de\u30eb\u30ad\u30f3\u30ab\u30f3\u3001C\uff1a\u30de\u30e1\u30ad\u30f3\u30ab\u30f3\u3001D\uff1a\u30cb\u30f3\u30dd\u30a6\u30ad\u30f3\u30ab\u30f3\u3001E\uff1a\u30ca\u30ac\u30df\u30ad\u30f3\u30ab\u30f3\u3001F\uff1a\u30d5\u30af\u30b7\u30e5\u30a6\u30ad\u30f3\u30ab\u30f3<\/span>\n<\/li>\n<li>\u500d\u6570\u6027\u5468\u7e01\u30ad\u30e1\u30e9\u7a81\u7136\u5909\u7570\u306e\u30ad\u30f3\u30ab\u30f3\u80b2\u7a2e\u3078\u306e\u5229\u7528\u306e\u53ef\u80fd\u6027\u3092\u691c\u8a0e\u3059\u308b\u305f\u3081\u306b\uff0c\u30cb\u30f3\u30dd\u30a6\u30ad\u30f3\u30ab\u30f3\u54c1\u7a2e\u2018\u52c7\u7d05\u2019\u306e\u500d\u6570\u6027\u3068\u5f62\u614b\u7684\u7279\u6027\u306b\u3064\u3044\u3066\u8abf\u67fb\u3057\u305f\uff0e\u305d\u306e\u7d50\u679c\uff0c\u2018\u52c7\u7d05\u2019\u306f\u7d44\u7e54\u8d77\u6e90\u5c64\u306e\u7b2c\u2160\u5c64\u304c\u4e8c\u500d\u4f53\uff0c\u7b2c\u2161\u5c64\u3068\u7b2c\u2162\u5c64\u304c\u56db\u500d\u4f53\u3067\u3042\u308b\u500d\u6570\u6027\u5468\u7e01\u30ad\u30e1\u30e9\u3067\u3042\u308a\uff0c\u305d\u306e\u679c\u5b9f\u7279\u6027\u306f\uff0c\u7a2e\u5b50\u304c\u5c11\u306a\u304f\uff0c\u539a\u76ae\u3067\u3042\u308a\uff0c\u30ad\u30f3\u30ab\u30f3\u306b\u3068\u3063\u3066\u671b\u307e\u3057\u3044\u5f62\u8cea\u3067\u3042\u3063\u305f\uff0e\u4eca\u5f8c\uff0c\u4eba\u70ba\u7684\u306b\u500d\u6570\u6027\u5468\u7e01\u30ad\u30e1\u30e9\u3092\u4f5c\u51fa\u3059\u308b\u3053\u3068\u3067\uff0c\u512a\u826f\u306a\u30ad\u30f3\u30ab\u30f3\u54c1\u7a2e\u306e\u4f5c\u51fa\u304c\u3067\u304d\u308b\u3082\u306e\u3068\u671f\u5f85\u3055\u308c\u305f\uff0e\uff08\u5b89\u7530\u3089\uff0c2008\uff09\n<div class=\"center-box\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-87\" src=\"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kinkan03.png\" alt=\"\u56f3\u3000\u30cb\u30f3\u30dd\u30a6\u30ad\u30f3\u30ab\u30f3\u54c1\u7a2e\u2018\u52c7\u7d05\u2019\u306e\u500d\u6570\u6027\" width=\"248\" height=\"369\" srcset=\"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kinkan03.png 248w, https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kinkan03-202x300.png 202w\" sizes=\"auto, (max-width: 248px) 85vw, 248px\" \/> <span class=\"img-name\">\u56f3\u3000\u30cb\u30f3\u30dd\u30a6\u30ad\u30f3\u30ab\u30f3\u54c1\u7a2e\u2018\u52c7\u7d05\u2019\u306e\u500d\u6570\u6027<\/span><\/div>\n<\/li>\n<li>\u30ad\u30f3\u30ab\u30f3\u80b2\u7a2e\u306b\u304a\u3051\u308b\u7a2e\u9593\u304a\u3088\u3073\u5c5e\u9593\u4ea4\u96d1\u3092\u7528\u3044\u305f\u4ea4\u96d1\u80b2\u7a2e\u3092\u691c\u8a0e\u3059\u308b\u305f\u3081\u306b\uff0c\u30ca\u30ac\u30ad\u30f3\u30ab\u30f3\u3068\u305d\u306e\u4ed6\u306e\u30ad\u30f3\u30ab\u30f3\u5c5e5\u7a2e\u3068\u306e\u7a2e\u9593\u4ea4\u96d1\u304a\u3088\u3073\u2018\u6e05\u898b\u2019\u30bf\u30f3\u30b4\u30fc\u30eb\u3068\u30ad\u30f3\u30ab\u30f3\u5c5e6\u7a2e\u3068\u306e\u5c5e\u9593\u4ea4\u96d1\u3092\u884c\u3063\u305f\uff0e\u30ca\u30ac\u30ad\u30f3\u30ab\u30f3\u3068\u306e\u7a2e\u9593\u4ea4\u96d1\u306b\u304a\u3044\u3066\uff0c\u3059\u3079\u3066\u306e\u4ea4\u96d1\u7d44\u307f\u5408\u308f\u305b\u304b\u3089\u96d1\u7a2e\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u305f\uff0e\u307e\u305f\uff0c\u2018\u6e05\u898b\u2019\u30bf\u30f3\u30b4\u30fc\u30eb\u3068\u306e\u5c5e\u9593\u4ea4\u96d1\u3067\u306f\uff0c\u30de\u30eb\u30ad\u30f3\u30ab\u30f3\u3068\u30de\u30e1\u30ad\u30f3\u30ab\u30f3\u4ee5\u5916\u306e\u7d44\u307f\u5408\u308f\u305b\u306b\u304a\u3044\u3066\uff0c\u4ea4\u96d1\u5b9f\u751f\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u305f\uff0e\u305d\u306e\u3046\u3061\uff0c\u2018\u6e05\u898b\u2019\u30bf\u30f3\u30b4\u30fc\u30eb\u3068\u30cb\u30f3\u30dd\u30a6\u30ad\u30f3\u30ab\u30f3\u3068\u306e\u4ea4\u96d1\u304b\u3089\u5f97\u3089\u308c\u305f2\u5b9f\u751f\u306f\uff0c\u975e\u9084\u5143\u96c4\u6027\u914d\u5076\u5b50\u7531\u6765\u306e\u7570\u6570\u4f53\uff082n = 28\uff09\u3068\u4e09\u500d\u4f53\uff082n = 3x = 27\uff09\u306e\u5c5e\u9593\u96d1\u7a2e\u3067\u3042\u308b\u3053\u3068\u304c\u78ba\u8a8d\u3055\u308c\u305f\uff0e\u3055\u3089\u306b\uff0c\u3053\u308c\u3089\u306f\uff0c\u96c4\u6027\u4e0d\u7a14\u3068\u5358\u70ba\u7d50\u679c\u6027\u306b\u3088\u308b\u7121\u6838\u679c\u3092\u751f\u7523\u3059\u308b\u3053\u3068\u304c\u3067\u304d\uff0c\u679c\u5b9f\u306f\uff0c\u679c\u76ae\u3092\u542b\u3081\u3066\u9ad8\u3044\u7cd6\u542b\u91cf\u3092\u6709\u3057\u3066\u3044\u305f\uff0e\uff08Yasuda et al., 2010b\uff09\n<div class=\"center-box\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-90\" src=\"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kinkan04.jpg\" alt=\"\u56f3\u3000\u2018\u6e05\u898b\u2019\u30bf\u30f3\u30b4\u30fc\u30eb\u3068\u30cb\u30f3\u30dd\u30a6\u30ad\u30f3\u30ab\u30f3\u3068\u306e\u5c5e\u7c21\u96d1\u7a2e\" width=\"455\" height=\"290\" srcset=\"https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kinkan04.jpg 455w, https:\/\/www.cc.miyazaki-u.ac.jp\/plantbreeding\/plant-wp\/wp-content\/uploads\/2016\/01\/kinkan04-300x191.jpg 300w\" sizes=\"auto, (max-width: 455px) 85vw, 455px\" \/> <span class=\"img-name\">\u56f3\u3000\u2018\u6e05\u898b\u2019\u30bf\u30f3\u30b4\u30fc\u30eb\u3068\u30cb\u30f3\u30dd\u30a6\u30ad\u30f3\u30ab\u30f3\u3068\u306e\u5c5e\u7c21\u96d1\u7a2e<\/span><\/div>\n<\/li>\n<\/ol>\n<ol class=\"intro-list\">\n<li>1\uff09\u5b89\u7530\u559c\u4e00\u3001\u570b\u6b66\u4e45\u767b\u3001\u4e2d\u5ddd\u5320\u5b50\u3001\u9ed2\u6728\u5b8f\u61b2\u3001\u516b\u5e61\u660c\u7d00\u3001\u5e73\u7530\u529b\u4e5f\u3001\u5409\u5009\u5e78\u535a\u3001\u5ddd\u4e0a\u90c1\u592b\u3001\u6749\u672c\u5b89\u5bdb\u300c\u30cb\u30f3\u30dd\u30a6\u30ad\u30f3\u30ab\u30f3\u2018\u52c7\u7d05\u2019\u306e\u500d\u6570\u6027\u5468\u7e01\u30ad\u30e1\u30e9\u306e\u8a3c\u660e\u3068\u305d\u306e\u5f62\u614b\u7684\u7279\u6027\u300d\u5712\u82b8\u5b66\u7814\u7a76\u30017\u5dfb2\u53f7\u3001165-171\u9801\u30012008<\/li>\n<li>2\uff09Yasuda K., M. Yahata, H. Komatsu and H. Kunitake.\u3000Phylogeny and classification of <em>Fortunella <\/em>(Aurantioideae) inferred from DNA polymorphism. Bulletin of the Faculty of Agriculture, University of Miyazaki, 56:103-110, 2010a\u3000<\/li>\n<li>3\uff09Yasuda K., M. Yahata, H. Komatsu, Y. Kurogi, and H. Kunitake. Triploid and Aneuploid Hybrids from Diploid-Diploid Intergeneric Crosses between Citrus Cultivar \u2018Kiyomi\u2019 Tangor and Meiwa kumquat (<em>Fortunella crassifolia<\/em> Swingle) for Seedless Breeding of Kumquat. J Japan Soc Hort Sci., 79(2):129-134 , 2010b<\/li>\n<li>4\uff09Yasuda, K., M. Yahata, M. Shigyo, R. Matsumoto, T. Yabuya and H. Kunitake. Identification of Parental Chromosomes in Sexual Intergeneric Hybrid Progenies between Citrus Cultivar \u2018Nanpu\u2019 Tangor and <em>Citropsis schweinfurthii<\/em> in the Subfamily Aurantioideae. J Japan Soc Hort Sci., 79(1):16-22 , 2010c<\/li>\n<li>5\uff09Yasuda, K., M. Yahata, H. Kunitake Phylogeny and Classification of Kumquats (Fortunella spp.) Inferred from CMA Karyotype Composition. 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