井口 純（いぐち あつし）
宮崎大学 農学部 畜産草地科学科
衛生微生物学/井口研究室
英文論文
2023
[61] Iguchi A* et. al. Genomic characterization of endemic diarrheagenic Escherichia coli and Escherichia albertii from infants with diarrhea in Vietnam. PLOS Neglected Tropical Diseases (accepted)
[60] Suzuki Y*, Shimizu H, Tamai S, Hoshiko Y, Maeda T, Nukazawa K, Iguchi A, Masago Y, Ishii S. Simultaneous detection of various pathogenic Escherichia coli in water by sequencing multiplex PCR amplicons. Environmental Monitoring and Assessment. 195(2):264 (2023)
2022
[59] Murase K, Arakawa E, Izumiya H, Iguchi A, Takemura T, Kikuchi T, Nakagawa I, Thomson NR, Ohnishi M, Morita M*. Genomic dissection of the Vibrio cholerae O-serogroup global reference strains: reassessing our view of diversity and plasticity between two chromosomes. Microbial Genomics. 8(8):000860 (2022)
[58] Ono T, Taniguchi I, Nakamura K, Nagano DS, Nishida R, Gotoh Y, Ogura Y, Sato MP, Iguchi A, Murase K, Yoshimura D, Itoh T, Shima A, Dubois D, Oswald E, Shiose A, Gotoh N, Hayashi T*. Global population structure of the Serratia marcescens complex and identification of hospital-adapted lineages in the complex. Microbial Genomics. 8(3):000793 (2022)
[57] Habets A, Touzain F, Lucas P, Huong NTT, Iguchi A, Crombé F, Korsak N, Piérard D, Saulmont M, Cox E, Engelen F, Mainil J, Thiry D*. Identification of Five Serotypes of Enteropathogenic Escherichia coli from Diarrheic Calves and Healthy Cattle in Belgium and Comparative Genomics with Shigatoxigenic E. coli. Veterinary Science. 9(9):492. (2022)
2021
[56] Nguyen TTH, Kikuchi T, Tokunaga T, Iyoda S, Iguchi A*. Diversity of the tellurite resistance gene operon in Escherichia coli. Frontiers in Microbiology 12:681175 (2021)
[55] Okuno K, Awasthi SP, Kopprio GA, Iguchi A, Hatanaka N, Hinenoya A, Lara RJ, Yamasaki S*. Prevalence, O-genotype and Shiga toxin (Stx) 2 subtype of Stx-producing Escherichia coli strains isolated from Argentinean beef cattle. Journal of Veterinary Medical Science. 83(4):630-636 (2021)
[54] Le PQ, Awasthi SP, Hatanaka N, Hinenoya A, Hassan J, Ombarak RA, Iguchi A, Tran NTT, Dao KVT, Vien MQ, Le HX, Do HT, Yamamoto Y, Yamasaki S*. Prevalence of mobile colistin resistance (mcr) genes in extended-spectrum β-lactamase-producing Escherichia coli isolated from retail raw foods in Nha Trang, Vietnam. International Journal of Food Microbiology. 346:109164 (2021)
[53] Lee K*, Iguchi A, Uda K, Matsumura S, Miyairi I, Ishikura K, Ohnishi M, Seto J, Ishikawa K, Konishi N, Obata H, Furukawa I, Nagaoka H, Morinushi H, Hama N, Nomoto R, Nakajima H, Kariya H, Hamasaki M, Iyoda S. Whole-Genome Sequencing of Shiga Toxin-Producing Escherichia coli OX18 from a Fatal Hemolytic Uremic Syndrome Case. Emerging Infectious Diseases. 27(5):1509-1512 (2021)
[52] Huong TTN, Iguchi A*, Ohata R, Kawai H, Ooka T, Nakajima H, Iyoda S. Distribution of novel Og-types in Shiga toxin-producing Escherichia coliisolated from healthy cattle. J Clin Microbiol. 59(3):e02624-20 (2021) (IF=5.897)
[51] Prah I, Ayibieke A, Huong NTT, Iguchi A, Mahazu S, Sato W, Hayashi T, Yamaoka S, Suzuki T, Iwanaga S, Ablordey A, Saito R*. Virulence profile of diarrhoeagenic Escherichia coli from the Western region of Ghana. Jpn J Infect Dis. 74(2):115-121 (2021)
2020
[50] Iguchi A*, Nishii H, Seto K, Mitobe J, Lee K, Konishi N, Obata H, Kikuchi T, Iyoda S. Additional Og-Typing PCR Techniques Targeting Escherichia coli-Novel and Shigella-Unique O-Antigen Biosynthesis Gene Clusters. J Clin Microbiol. 2020 Oct 21;58(11):e01493-20. (IF=5.897)
[49] Jikumaru A, Ishii S, Fukudome T, Kawahara Y, Iguchi A, Masago Y, Nukazawa K, Suzuki Y. Fast, sensitive, and reliable detection of waterborne pathogens by digital PCR after coagulation and foam concentration. J Biosci Bioeng. 2020 Jul;130(1):76-81. doi: 10.1016/j.jbiosc.2020.02.004.
2019
[48] rimizu Y, Kirino Y, Sato MP, Uno K, Sato T, Gotoh Y, Auvray F, Brugere H, Oswald E, Mainil JG, Anklam KS, Döpfer D, Yoshino S, Ooka T, Tanizawa Y, Nakamura Y, Iguchi A, Morita-Ishihara T, Ohnishi M, Akashi K, Hayashi T, Ogura Y*.
Genome Res. 2019 Sep;29(9):1495-1505. doi: 10.1101/gr.249268.119.
2018
[47] Banjo M, Iguchi A*, Seto K, Kikuchi T, Harada T, Scheutz F, Iyoda S; Pathogenic E. coli Working Group in Japan. Escherichia coli H-genotyping PCR; a complete and practical platform for molecular H-typing. J Clin Microbiol. 2018 Mar 28. pii: JCM.00190-18. doi: 10.1128/JCM.00190-18.
[46] Nakamura H, Iguchi A, Maehara T, Fujiwara K, Fujiwara A, Ogasawara J. Comparison of Three Molecular Subtyping Methods among O157 and Non-O157 Shiga Toxin-Producing Escherichia coli Isolates from Japanese Cattle. Jpn J Infect Dis. 2018 Jan 23;71(1):45-50. doi: 10.7883/yoken.JJID.2017.297.
2017
[45] Thiry D, Saulmont M, Takaki S, De Rauw K, Duprez JN, Iguchi A, Piérard D, Mainil JG. Enteropathogenic Escherichia coli O80:H2 in Young Calves with Diarrhea, Belgium. Emerg Infect Dis. 2017 Dec;23(12):2093-2095.
[44] Suzuki Y, Niina K, Matsuwaki T, Nukazawa K, Iguchi A. Bacterial flora analysis of coliforms in sewage, river water, and ground water using MALDI-TOF mass spectrometry. Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering. 2017 Nov 17:1-14.
[43] Iguchi A*, Mentzer von A, Kikuchi T, Thomson R N. An untypeable enterotoxigenic Escherichia coli represents one of the dominant types causing human disease. Microbial Genomics 2017 Jul 3;3(9):e000121.
[42] Kabeya H*, Sato S, Oda S, Kawamura M, Nagasaka M, Kuranaga M, Yokoyama E, Hirai S, Iguchi A, Ishihara T, Kuroki T, Morita-Ishihara T, Iyoda S, Terajima J, Ohnishi M, Maruyama S. Characterization of Shiga toxin-producing Escherichia coli from feces of sika deer (Cervus nippon) in Japan using PCR binary typing analysis to evaluate their potential human pathogenicity. Journal of Veterinary Medical Science. 2017 May 3;79(5):834-841.
[41] Fakih I, Thiry , Duprez JN, Saulmont M, Iguchi A, Piérard D, Jouant L, Daube G, Ogura Y, Hayashi T, Taminiau B, Mainil JG*. Identification of Shiga toxin-producing (STEC) and enteropathogenic (EPEC) Escherichia coli in diarrhoeic calves and comparative genomics of O5 bovine and human STEC Veterinary Microbiology 2017 Apr;202:16-22.
[40] Akiyama Y*, Futai H, Saito E, Ogita K, Sakae H, Fukunaga M, Tsuji H, Chikahira M, Iguchi A. Shiga Toxin Subtypes and Virulence Genes Distributed among Escherichia coli Isolated from Cattle. Japanese Journal of Infectious Diseases.
2017 Mar 24;70(2):181-185.
[39] Ishijima N, Lee KI, Kuwahara T, Nakayama-Imaohji H, Yoneda S, Iguchi A, Ogura Y, Hayashi T, Ohnishi M, Iyoda S. Identification of a New Virulent Clade in Enterohemorrhagic Escherichia coli O26:H11/H- Sequence Type 29. Scientific Reports. 2017 Feb 23;7:43136.
2016
[38] Morita-Ishihara T*, Iyoda S, Iguchi A, Ohnishi M Secondary Shiga Toxin-Producing Escherichia coli Infection, Japan, 2010-2012. Emerging Infectious Disease 22(12):2181-2184 (2016)
[37] Imuta N, Ooka T, Seto K, Kawahara R, Koriyama T, Kojyo T, Iguchi A, Tokuda K, Kawamura H, Yoshiie K, Ogura Y, Hayashi T, Nishi J*. Phylogenetic analysis of enteroaggregative Escherichia coli (EAEC) isolated in Japan revealed the emergence of CTX-M-14-producing EAEC O25:H4 clones related to ST131. Journal of Clinical Microbiology 54(8):2128-34 (2016)
[36] Iguchi A*, Iyoda S, Seto K, Nishii H, Ohnishi M, Mekata H, Ogura Y, Tetsuya H. Six novel O genotypes from Shiga toxin-producing Escherichia coli. Frontiers in Microbiology 20;7:765. (2016) IF2014=3.989
[35] Ombarak RA, Hinenoya A, Awasthi SP, Iguchi A, Shima A, Elbagory AR, Yamasaki S*. Prevalence and pathogenic potential of Escherichia coli isolates from raw milk and raw milk cheese in Egypt. International Journal of Food Microbiology 16;221:69-76. (2016)
2015
[34] Harada T*, Iguchi A, Iyoda S, Seto K, Taguchi M, Kumeda Y. Multiplex Real-Time PCR Assays for Screening of Shiga Toxin 1 and 2 Genes, Including All Known Subtypes, and Escherichia coli O26-, O111-, and O157-Specific Genes in Beef and Sprout Enrichment Cultures. Journal of Food Protection 78(10):1800-11 (2015)
[33] Joensen K, Tetzschner A, Iguchi A, Aarestrup F, Scheutz F*. Rapid and easy in silico serotyping of Escherichia coli using Whole genome sequencing (WGS) data. Journal of Clinical Microbiology 53:2410-26 (2015)
[32] Iguchi A*, Iyoda S, Seto K, Morita-Ishihara T, Scheutz F, Ohnishi M, and Pathogenic E. coli Working Group in Japan. Escherichia coli O-genotyping PCR; a comprehensive and practical platform for molecular O-serogrouping. Journal of Clinical Microbiology 53(8):2427-32 (2015) IF2013=4.232
[31] Iguchi A*, Iyoda S, Kikuchi T, Ogura Y, Katsura K, Ohnishi M, Hayashi T, Thomson NR. A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster. DNA Research 22(1):101-7 (2015) IF2013=4.975 2014/11/04 up
[30] Nishiyama M, Iguchi A, Suzuki Y*. Identification of Enterococcus faecium and Enterococcus faecalis as vanC-type vancomycin-resistant enterococci (VRE) from sewage and river water in the provincial city of Miyazaki, Japan. Journal of Environmental Science and Health, Part A Toxic/Hazardous Substances and Environmental Engineering 50(1):16-25 (2015) 2014/08/07 up
[29] von Mentzer A*, Connor T, Wieler LH, Semmler T, Iguchi A, Thomson NR, Rasko DA, Joffre E, Corander J, Pickard D, Wiklund G, Svennerholm A, Sjöling A, Dougan G. Identification of enterotoxigenic Escherichia coli (ETEC) clades with significant long-term global distribution. Nature Genetics 46(12):1321-6 (2014) 2014/10/20 up
2014
[28] Iguchi A, Nagaya Y, Pradel E, Ooka T, Ogura Y, Katsura K, Kurokawa K, Oshima K, Hattori M, Parkhill J, Sebaihia M, Coulthurst SJ, Gotoh N, Thomson NR*, Ewbank JJ, Hayashi T*. Genome Evolution and Plasticity of Serratia marcescens, an Important Multidrug-Resistant Nosocomial Pathogen. Genome Biology and Evolution 6(8):2096-2110 (2014) IF2013=4.532 2014/08/07 up
[27] Iyoda S*, Manning SD, Seto K, Kimata K, Isobe J, Etoh Y, Ichihara S, Migita Y, Ogata K, Honda M, Kubota T, Kawano K, Matsumoto K, Kudaka J, Asai N, Yabata J, Tominaga K, Terajima J, Morita-Ishihara T, Izumiya H, Ogura Y, Saitoh T, Iguchi A, Kobayashi H, Hara-Kudo Y, Ohnishi M, and EHEC working group in Japan. Phylogenetic clades 6 and 8 of enterohemorrhagic Escherichia coli O157:H7 with particular stx subtypes are more frequently found in isolates from hemolytic uremic syndrome patients than from asymptomatic carriers. Open Forum Infectious Diseases 1(2): ofu061 (2014) 2014/08/07 up
[26] Honda A*, Hirose M, Yasmin L, Yuzawa K, Honsho K, Izu H, Iguchi A, Ikawa M, Ogura A. Single-step generation of rabbits carrying a targeted allele of Tyrosinase gene using CRISPR/Cas9. Experimental Animals 64(1):31-7 (2015) 2014/08/07 up
[25] Mekata H, Iguchi A, Kawano K, Kirino Y, Kobayashi I, Misawa N. Identification of O-serotypes, -genotypes and virulotypes of Shiga toxin-producing Escherichia coli isolates including non-O157 from beef cattle in Japan. Journal of food protection 77(8):1269-1274 (2014) 2014/08/07 up
〜2013年度
[24] Osawa K, Shigemura K, Iguchi A, Shirai H, Imayama T, Seto K, Raharjo D, Fujisawa M, Osawa R, Shirakawa T*. O-antigen chain length modulated by the wzz gene in Escherichia coli O157 influenced its sensitivities to serum complement. Microbiology and immunology. 57(9): 616-623 (2013)
[23] Inoue K*, Ito T, Kawano Y, Iguchi A, Miyahara M, Suzuki Y, Watanabe K. Electricity generation from cattle manure slurry by cassette-electrode microbial fuel cells. Journal of bioscience and bioengineering. S1389-1723(13)00174-6. (2013)
[22] Suzuki Y*, Kajii S, Nishiyama M, Iguchi A. Susceptibility of Pseudomonas aeruginosa isolates collected from river water in Japan to antipseudomonal agents. Science of The Total Environment. 15;450-451:148-54. (2013)
[21] Iguchi A*, Iyoda S, Ohnishi M, on behalf of the EHEC Study Group. Molecular Characterization Reveals Three Distinct Clonal Groups among Clinical Shiga Toxin-Producing Escherichia coli of Serogroup O103. Journal of Clinical Microbiology 50(9):2894-900 (2012) IF(2010)=4.220
[20] Murase K, Ooka T, Iguchi A, Ogura Y, Nakayama K, Asadulghani M, Islam MR, Hiyoshi H, Kodama T, Beutin L, Hayashi T*. Hemolysin E- and enterohemolysin-derived hemolytic activity of O55/O157 strains and other Escherichia coli lineages. Microbiology 158:746-58 (2012)
→ PubMed
[19] Iguchi A*, Iyoda S, Seto K, Ohnishi M, on behalf of the EHEC Study Group. Emergence of a Novel Shiga Toxin-Producing Escherichia coli O-serogroup Cross-reacting with Shigella boydii Type 10. Journal of Clinical Microbiology 49(10): 3678-3680 (2011) IF(2010)=4.220
→ PubMed
[18] Iguchi A*, Shirai H, Seto K, Ooka T, Ogura Y, Hayashi T, Osawa K, Osawa R, Wide Distribution of O157-antigen Biosynthesis Gene Clusters in Escherichia coli. PLoS ONE 6(8):e23250 (2011) IF(2010)=4.411
[17] Iguchi A, Umekawa N, Maegawa T, Turuta H, Odamaki T, Xiao J, Osawa R*, Polymorphism and distribution of putative cell-surface adhesin-encoding ORFs among human fecal isolates of Bifidobacterium longum subsp. longum. Antonie van Leeuwenhoek 99(3):457-71 (2011) IF(2009)=1.983
→ PubMed
[16] Hilo Yen, Ooka T, Iguchi A, Hayashi T, Sugimoto N, Tobe*, NleC, a type III secretion protease, compromises NF-κB activation by targeting p65/RelA. PLoS Pathogen 16;6(12):e1001231 (2010)
→ PubMed
[15] Ogura Y, Ooka T, Iguchi A, Toh H, Asadulghani M, Oshima K, Kodama T, Abe H, Nakayama K, Kurokawa K, Tobe T, Hattori M, Hayashi T*, Comparative genomics reveal the mechanism of the parallel evolution of O157 and non-O157 enterohemorrhagic Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 106(42): 17939-17944 (2009)
[14] Ooka T, Terajima J, Kusumoto M, Iguchi A, Kurokawa K, Ogura Y, Asadulghani M, Nakayama K, Murase K, Ohnishi M, Iyoda S, Watanabe H, Hayashi T*, Development of a multiplex PCR-based rapid typing method for enterohemorrhagic Escherichia coli O157 strains. Journal of Clinical Microbiology 47(9): 2888-2894 (2009)
[13] Asadulghani M, Ogura Y, Ooka T, Itoh T, Sawaguchi A, Iguchi A, Nakayama K, Hayashi T*. The defective prophage pool of Escherichia coli O157: prophage-prophage interactions potentiate horizontal transfer of virulence determinants. PLoS Pathogen 5(5):e1000408 (2009)
[12] Leopold S R, Magrini V, Holt J N, Shaikh N, Mardis R E, Cagno J, Ogura Y, Iguchi A, Hayashi T, Mellmann A, Karch H, Besser E T, Sawyer A S, Whittam S T, Tarr I P*, A precise reconstruction of the emergence and constrained radiations of Escherichia coli O157 portrayed by backbone concatenomic analysis. Proceedings of the National Academy of Sciences of the United States of America 106(21): 8713-8718 (2009)
[11] Iguchi A, Thomson N R, Ogura Y, Saunders D, Ooka T, Henderson I R, Harris D, Asadulghani M, Kurokawa K, Dean P, Kenny B, Quail M A, Thurston S, Dougan G, Hayashi T, Parkhill J, Frankel G*, Complete genome sequence and comparative genome analysis of enteropathogenic E. coli O127:H6 strain E2348/69. Journal of Bacteriology, 191(1): 347-354 (2009)
[10] Ogura Y, Abe H, Katsura K, Kurokawa K, Asadulghani M, Iguchi A, Ooka T, Nakayama K, Yamashita A, Hattori M, Tobe T, Hayashi T*, Systematic identification and sequence analysis of the genomic islands of the enteropathogenic Escherichia coli strain B171-8 by the combined use of Whole Genome PCR Scanning and fosmid mapping. Journal of Bacteriology 190(21): 6948-6960 (2008)
[9] Iguchi A, Ooka T, Ogura Y, Asadulghani, Nakayama K, Frankel G, Hayashi T*, Genomic comparison of the O-antigen biosynthesis gene clusters of Escherichia coli O55 strains belonging to three distinct lineages. Microbiology 154: 559-570 (2008)
[8] Iguchi A, Iyoda S, Watanabe H, Osawa R*, O side chain deficiency enhances sensitivity of Escherichia coli to Shiga toxin 2-converting bacteriophages. Current Microbiology 54(1): 14-19 (2007)
[7] Iguchi A, Iyoda S, Terajima J, Watanabe H, Osawa R*, Spontaneous recombination between homologous prophage regions causes large-scale inversions within the Escherichia coli O157:H7 chromosome. Gene 10(372): 199-207 (2006)
[6] Okura M, Osawa R*, Iguchi A, Takagi M, Arakawa E, Terajima J, Watanabe H, PCR-based identification of pandemic group Vibrio parahaemolyticus with a novel group-specific primer pair. Microbiology and Immunology 48(10): 787-790 (2004)
→PubMed
[5] Okura M, Osawa R*, Iguchi A, Arakawa E, Terajima J, Watanabe H, Genotypic analyses of Vibrio parahaemolyticus and development of a pandemic group specific multiplex PCR assay. Journal of Clinical Microbiology 41(10): 4676-4682 (2003)
→PubMed
[4] Sata S, Fujisawa T, Osawa R*, Iguchi A, Yamai S, Shimada T, An improved enrichment broth for isolation of Escherichia coli O157, with specific reference to starved cells, from radish sprouts. Applied and Environmental Microbiology 69(3): 1858-1860 (2003)
→PubMed
[3] Iguchi A, Osawa R*, Kawano J, Shimizu A, Terajima J, Watanabe H, Effects of lysogeny of Shiga toxin 2-encoding bacteriophages on pulsed-field gel electrophoresis fragment pattern of Escherichia coli K-12. Current Microbiology 46(3): 224-227 (2003)
→PubMed
[2] Iguchi A, Osawa R*, Kawano J, Shimizu A, Terajima J, Watanabe H, Effects of repeated subculturing and prolonged storage at room temperature of enterohemorragic Escherichia coli O157:H7 on pulsed-field gel electrophoresis profile. Journal of Clinical Microbiology 40(8): 3079-3081 (2002)
→PubMed
[1] Osawa R*, Iguchi A, Arakawa E, Watanabe H, Genotyping of pandemic Vibrio parahaemolyticus O3:K6 still open to question. Journal of Clinical Microbiology 40(7): 2708-2709 (2002)
→PubMed
日本語論文・報告・総説など
[事例報告] HUS患者から分離されたstx2f陽性のEscherichia albertiiについて 伊豫田淳ら10名 病原微生物検出情報(IASR) Vol. 37 p.255: 2016年12月号
[マニュアル] 腸管出血性大腸菌（EHEC） 検査・診断マニュアル 2017年2月改訂 執筆者：伊豫田淳ら10名 国立感染症研究所 病原体検出マニュアル
[総説] 大腸菌のO抗原合成遺伝子領域の解析と検査法の確立 井口純 日本細菌学雑誌 Vol. 71 No.4 p. 209-215 (2016)
[原著論文] さまざまなO血清群に属する志賀毒素産生性大腸菌の市販選択培地上での生育特性 秋吉充子、中村寛海、伊豫田淳、石原朋子、加藤結子、井口純 日本食品微生物学会雑誌 32(4):192‒198 (2015)
[原著論文] 腸管出血性大腸菌の主要なO血清群と病原性遺伝子を 判定するOne-shotマルチプレックスPCR法の開発と評価 井口純、秋吉充子、伊豫田淳、大西真 日本食品微生物学会雑誌 32(4):215‒218 (2015)
● 研究論文（* ; corresponding author）